How many larvae to sample?
Larval and juvenile fish cohorts often have skewed size distributions due to interindividual variation in growth rates.
A resampling analysis was used to investigate how much sampling effort is required to achieve repeatable average size estimates in populations of juvenile yellowtail kingfish Seriola lalandi. One hundred juveniles (30 days post-hatch) were measured for total length and wet weight from 9 cohorts reared in captivity. From an analysis of cohort size distribution the mean was determined to be a suitable measure of the average for length, while the median was best for weight. One thousand resamples of mean length and median weight were performed for each cohort data set, with sample sizes ranging from 2 to 100 individuals. The point at which increasing sample size resulted in only a negligible increase in precision (variance between resamples <0.1%) corresponded to n = 19 for mean length and n = 30 for median weight.
Since yellowtail kingfish display a magnitude of size heterogeneity similar to other marine species with small pelagic larvae reared in captivity, the sample sizes determined in this study can act as a general guideline for larval fish studies.
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This work was made possible by a postdoc fellowship from the NZ Foundation for Research Science and Technology and a Marie Curie Fellowship
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This research has been published and is available as follows:
Moran, D., (2011). Analysis of sampling effort required to obtain repeatable average size estimates of juvenile fish. Aquatic Biology 11,239-242.
doi:10.3354/ab00316 ------- get PDF of paper